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This 6 day course is part of the outreach and training activities of the NCRR National Resource for Automated Molecular Microscopy (NRAMM), located at the Center for Integrative Molecular Biosciences (CIMBio) at The Scripps Research Institute (TSRI). The course is a follow on from previous training courses in cryoEM held at NRAMM in 2003, 2005 and 2007 (see Workshops). This year we are also partnering with the Joint Center for Innovative Protein Technologies (JCIMPT) to address problems of mutual interest to the scientific communities supported by both of these NCRR supported Resources. The course is aimed primarily at students and postdocs who have already received basic training and have some initial experience practicing cryoEM.
A major goal of the course will be to discuss some of the challenges we face in using cryoEM to solve macromolecules that are either small or conformationally variable in structure, and which thus present unique challenges to the existing techniques. In cooperation with JCIMPT, one day of the course will focus specifically on membrane proteins. Topics to be discussed will include improvements in specimen preparation, imaging, processing and reconstruction. The basic format of the course will be theoretical lectures in the mornings followed by practical demonstrations and panel discussions in the afternoon. Evenings will be reserved for poster presentations by student participants and research talks by instructors.
Bridget Carragher, Clinton S. Potter, Ronald A Milligan
National Resource for Automated Molecular Microscopy
The Scripps Research Institute