Welcome to NRAMM
One of the major goals for the current five year grant period is to expand our automated infrastructure in order to provide a complete and integrated pipeline for the reconstruction of macromolecular machines. This infrastructure is primarily aimed at supporting the overall NRAMM mission of developing cryoEM as a mainstream and efficient methodology on a par with, and complementary to, X-ray crystallography. An additional major goal of the core technological and research developments is to address some of the fundamental questions and issues that remain as barriers to deriving high resolution 3D maps of molecular machines using cryoEM. The technology that we are developing provides us with a unique opportunity to systematically probe some of the current limitations of the method.
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“Computing and controls at Diamond Light Source”
Nick Rees, Principal Software Engineer, Diamond Light Source
Thursday October 3, 2013 @12:00 p.m. CIMBio 1st Floor Conference Room
Diamond Light Source is the UK National Synchrotron facility and is now approaching the end of its seventh year of operation with over 20 beamlines in operation and funding for 33. It has taken the approach of providing central support groups for a lot of the core disciplines, rather than depending on beamline staff and funding to provide services for each beamline. This talk introduces Diamond, and describes the challenges faced by the support teams, focussing particularly on the computing and controls challenges posed by modern high rate detectors. In particular, the IT infrastructure at Diamond is world leading within the synchrotron community and is designed to handle the data rate from many beamlines generating data at 10 GbE speeds simultaneously.
Recent publications acknowledging NRAMM:
Bailly X, Reichert H, Hartenstein V. The urbilaterian brain revisited: novel insights into old questions from new flatworm clades. Dev Genes Evol. 2013;223(3):149-57.
Cheng A. Automation of data acquisition in electron crystallography. Methods Mol Biol. 2013;955:307-12.
Cheng A. Automated grid handling and image acquisition for two-dimensional crystal screening. Methods Mol Biol. 2013;955:297-305.
Cui H, Mim C, Vazquez FX, Lyman E, Unger VM, Voth GA. Understanding the Role of Amphipathic Helices in N-BAR Domain Driven Membrane Remodeling. Biophys J. 2013;104(2):404-11.
Gogol EP, Akkaladevi N, Szerszen L, Mukherjee S, Chollet-Hinton L, Katayama H, Pentelute BL, Collier RJ, Fisher MT. Three dimensional structure of the anthrax toxin translocon-lethal factor complex by cryo-electron microscopy. Protein Sci. 2013;22(5):586-94.
Julien JP, Lee JH, Cupo A, Murin CD, Derking R, Hoffenberg S, Caulfield MJ, King CR, Marozsan AJ, Klasse PJ, Sanders RW, Moore JP, Wilson IA, Ward AB. Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9. Proc Natl Acad Sci U S A. 2013;110(11):4351-6.
See publications for a complete list…